Downregulation of pERK was also observed, suggesting that similarly to other cell types (hepatoma cells, CHO‐K1, and glioma cells) EDG5 in hESCs may transduce its signal via G12/13 and Gi subunits, which signal and participate in pathway leading to activation of the MAPK protein ERK 36, 38. This evidence concerns the gene EIF2AK3 and hepatocellular carcinoma.