FOXO1 and hydrops fetalis: Subsequent gene expression analysis showed that EC-FoxO1 depletion did not change transcript levels of Pecam1 in the liver (corresponding with the lack of Cre recombination in this organ) but did upregulate its expression in skeletal muscle, suggesting that under the stimulus of HF diet, EC-FoxO1 depletion also induces microvascular remodeling in this tissue (Figure 4A).