In addition, increasing HIF-1α transcriptional activity and iNOS mRNA expression by inhibition of HIF-1α-prolyl-4 hydroxylase-2 (PHD2) gene expression significantly reduced myocardial infarction size in mice with ischemia reperfusion, suggesting that the activation of HIF-1α in hearts by PHD2 siRNA administration attenuated reperfusion injury through an iNOS-dependent pathway [62]. Here, NOS2 is linked to myocardial infarction.