We then evaluated the cytolytic activity of CAR T cells after 72 h of co-culture with freshly thawed patient primary ovarian cancer ascites, and showed potent and selective CAR-mediated killing of the TAG72-positive OAS4 and OAS7 cells, with no detectable anti-tumor activity against the TAG72-negative OAS3 cells (Figure 2G). Here, OAS3 is linked to ovarian cancer.