NR3C1 and major depressive disorder: Subjects: patients with BPD (n = 101, 95 female), MDD (n = 99, with low rate of abuses and neglect) and MDD with comorbid PTSD (n = 15). All subjects received psychotropic medication. Diagnostics: DSM-IV, SCID-II, DGIS, CTQ, BDI-II, clinical interview for detailed characteristics of suffered abuse. Methods: DNA bisulfite treatment, PCR amplification and pyrosequencing for selected methylation analysis of NR3C1 exon 1F (8 CpG sites). Sample validation by confirmation analyses.