Subjects: patients with BPD (n = 101, 95 female), MDD (n = 99, with low rate of abuses and neglect) and MDD with comorbid PTSD (n = 15). All subjects received psychotropic medication. Diagnostics: DSM-IV, SCID-II, DGIS, CTQ, BDI-II, clinical interview for detailed characteristics of suffered abuse. Methods: DNA bisulfite treatment, PCR amplification and pyrosequencing for selected methylation analysis of NR3C1 exon 1F (8 CpG sites). Sample validation by confirmation analyses. This evidence concerns the gene NR3C1 and post-traumatic stress disorder.