To determine the polarization status of general macrophage populations in the tumors and their changes with injection of USPIO, immunofluorescence staining of the tumor cross sections was conducted for pan-macrophage (CD68), inflammatory (M1-like AIF1 (allograft inflammatory factor 1)), and wound-healing surface marker phenotypes (M2-like CD206 (mannose receptor)), besides Prussian blue iron histology as the primary observable (Figures 3–3). The gene discussed is AIF1; the disease is neoplasm.