To confirm the results obtained with C2C12 cells, we injected Wnt3a into the TA muscles of control and Islr cKO mice after CTX injury, and found that Wnt3a could not effectively activate canonical Wnt signaling during skeletal muscle regeneration (Fig. 7c, d), which is consistent with the results of C2C12 cells. The gene discussed is ISLR; the disease is cerebrotendinous xanthomatosis.