Using a delta Ct threshold to the ACHE control region of 9 for the five markers ASTN1, DLX1, ITGA4, RXFP3, and SOX17 and a delta Ct threshold of 10 for ZNF671, we achieved a very good specificity (< 98%) in the NILM group combined with an excellent sensitivity for cancer cases (100%) and a detection rate for the different precancerous stages CIN1, 2 and 3 increasing with grade. Here, ASTN1 is linked to cancer.