There were 2673 differentially expressed mRNAs, and the top 10 upregulated and downregulated mRNAs are shown in Figure 1A. The intersection of the differentially expressed mRNAs and the genes involved in the KEGG NSCLC pathway included 9 mRNAs (RASSF1, GADD45B, PRKCB, GADD45G, STAT5A, PIK3R1, GADD45A, EGFR and E2F2). The gene discussed is RASSF1; the disease is non-small cell lung carcinoma.