In line with the current animal welfare regulations, we were able to identify a suitable dose of P. aeruginosa, which leads to a prominent infection in mice represented by a disease score of 6–8 in hIL-3/GM-CSF KI mice (0: unaffected, 10: moribund) within 24 h post infection without leading to mortality of the animals. The gene discussed is CSF2; the disease is infection.