EGFR and mesothelioma: In the cell binding assay, we used the A431 cell line that overexpresses EGFR on their surface (2 × 106 receptors by cell) [42], we found an elevated percentage of RIC binding efficiency in the cell line MSTO-211H (biphasic mesothelioma cells), with less expression of EGFR (34 vs. 21%).