The co-treatment of GCA and VP gradually reduced expression levels of Sox9 and the YAP target genes Cyr61, Ctgf, Afp and Ankrd1 (Figure 6B,D, Figure S6E,F) indicating a direct link between cholestasis mediated expression of Sox9 through activation of YAP, which is independent of RBPJ (Figure 5C). Here, CCN2 is linked to cholestasis.