Of the 34 validated mutations that were significantly enriched in the lung metastases, 17 were predicted to impact protein function (SIFT41 and PolyPhen42), and four fell in cancer genes (CSF3R_Q250L, CDH11_A683S, GRIN2A_S752R, and CDK8_H235Y; Fig. 2d and Supplementary Data 2). Here, CDK8 is linked to cancer.