Then, we detected the mRNA and protein expression of PIK3R1 in a series of human HCC cell lines, including MHCC97L, Huh7, HepG2, HCCLM3, SMMC-7721 and MHCC97H by qRT-PCR and Western Blot analyses, respectively. This evidence concerns the gene PIK3R1 and hepatocellular carcinoma.