TP53 and neoplasm: Consistently, transplantation experiments using established tumor organoids indicated that triple mutations of ApcΔ716KrasG12DTgfbr2−/− and ApcΔ716KrasG12DTrp53+/R270H are minimum cores for liver metastasis, but other double mutations or even triple mutation of ApcΔ716Trp53+/R270HTgfbr2−/− are not sufficient for the induction of metastasis, suggesting that a Kras mutation in addition to Wnt activation and TGF-β suppression or p53 mutation is critical for metastasis (Figure 3).