Specific siRNA depletion of many of the nucleoporins (confirmed by immunoblotting, S3 Fig) significantly reduced the magnitude of MX2 inhibition (Fig 3B); in particular, silencing of NUP214, NUP62, NUP153, NUP88, NUP358, NUP98 and transportins TNPO1 and TNPO3 each reduced the magnitude of MX2 inhibition of HIV-1 infection to below 3-fold. The gene discussed is TNPO3; the disease is HIV-1 infection.