Considering that both aging and CML induce changes in phenotype and function of NK cells, in this work we have studied the expression of several markers (CD11b, CD27, CD57, CD69, HLA-DR, NKG2A, NKG2C, NKG2D, NKp30, NKp44, NKp46, and NKp80) in NK cell subsets, and CD107a and IFN-γ in K562 stimulated NK cells, from CML patients and healthy controls, stratified according to age in middle-aged and elderly donors. Here, CD69 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.