Inspection of the GEO database (http://www.ncbi.nlm.nih.gov/geo/info/profiles.html) revealed that Ash1L expression is significantly downregulated in muscle tissue from both DMD patients and the DMD mouse model mdx, which we confirmed by real-time quantitative reverse transcription PCR (RT-qPCR) (Supplementary Figure 1). This evidence concerns the gene ASH1L and Duchenne muscular dystrophy.