Since a similar activation of p65 is observed in the three human NSCLC cell lines, we next selected A549 cells for subsequent experiments and exposed these for 3 h to the NF-κB upstream activator TNF-α and measured NF-κB transcriptional activation using a luciferase reporter (Wilson et al., 2013). The gene discussed is TNF; the disease is non-small cell lung carcinoma.