The inhibition of mTOR by temsirolimus increases the cytotoxic and pro-apoptotic activity of PENAO (an inhibitor of adenine nucleotide translocase) on DIPG (diffuse intrinsic pontine gliomas) cells through the generation of reactive oxygen species, ATP depletion, and an increase in the activity of AMPK, with the subsequent inhibition of the PDGFRa /PI3K/mTOR and HSP90 signaling pathways [272]. The gene discussed is MTOR; the disease is diffuse intrinsic pontine glioma.