In this regard, the results elicited the individual contribution of His37, His129, Glu75, Asp127, His85, Asn27 and Asn119 amino acid residues from carbohydrate-binding sites to CGL activity could be helpful for designing an artificial analog of CGL with enhanced Gb3- and mucin-binding properties for applying in cancer diagnostics or anticancer therapy. This evidence concerns the gene CTH and cancer.