In lung cancer, Sp1 could be repressed by miR‐29c, miR‐27b, miR‐335 and miR‐429 in translational level.66, 67, 68 Similar to these findings, our previous study revealed that miR‐326 was deterred in lung cancer cells, and transfection of miR‐326 significantly decreased cell proliferation and migration.44 Recently, the increasing studies have focused on the target genes of miR‐326 that could interact with miR‐326 to impede tumour development and progression, such as NOB1,11 phox2a,44 CCND1,69 FSCN115 and NSBP1.70 In the present study, we demonstrated that Sp1 is a downstream target of miR‐326. Here, PHOX2A is linked to lung cancer.