Furthermore, to investigate whether miR‐326 could regulate Sp1 and KLF3 expression, we transfected miR‐326 mimics into lung cancer cells and real‐time PCR results revealed that overexpression of miR‐326 suppressed the expression of Sp1 and KLF3 in both A549 (Figure 7E) and 95D (Figure 7F) cells. The gene discussed is KLF3; the disease is lung cancer.