In our preview studies, we have found that miR‐326 inhibited lung cancer cell proliferation and migration.44 Meanwhile, through bioinformatics analysis such as JASPAR and TargetScan, we found the 3′UTR regions of KLF3 and Sp1 contained the putative binding sites of miR‐326, suggesting Sp1 and KLF3 may be potential downstream targets of miR‐326. This evidence concerns the gene KLF3 and lung cancer.