After summarizing quantitative data for the top TMT-labelled phosphoproteins detected, our data show that alpha-2-HS-glycoprotein / fetuin-A is the most abundant phosphoprotein present in dog serum both in enriched and non-enriched pools (Fig 6) with around 50–70% of the total intensity corresponding to enriched phosphopeptides and no variation when comparing control and babesiosis pools. Here, AHSG is linked to babesiosis.