To better understand the role of down‐regulation of SPINT2/HAI‐2 in BMMSC isolated from MDS and de novo AML patients, we inhibited its expression using a lentiviral vector delivering shRNA specific to human SPINT2 gene, in an HS‐5 stromal cell line immortalized from a bone marrow of healthy donor. This evidence concerns the gene SPINT2 and acute myeloid leukemia.