In this study, several ASD-associated behaviors in Slit3-KO mice were examined, including motor behaviors (open field test and rotarod test), anxiety-like behaviors (light/dark box and elevated plus maze test), depression-like behaviors (tail suspension test), cognitive ability (novel object recognition test), social behaviors (three chamber test), and repetitive behaviors (marble burying test). The gene discussed is SLIT3; the disease is major depressive disorder.