Healthy donor blood spiked with cells of the prostate cancer cell line PC3 (1.0 × 104 EpCAM antigens, average size 17.7 μm) and the breast cancer cell line MDA-MB-231 (1.5 × 104 EpCAM antigens, average size 15.6 μm) were tested for recovery of EpCAMhigh and EpCAMlow cells. Here, EPCAM is linked to breast cancer.