BRAF and neoplasm: BRAF mutations were present in 26/347 tumors (7.5%) (Figure 2B); 21 had the classic p.Val600Glu (p.V600E); 4 had non-V600E mutations; and, 1 tumor had p.Val600Glu and p.Ala598Thr at respective variant allelic frequencies (VAFs) of 16% and 10% in a sample with 50% tumor cell DNA (Supplementary Table 2).