The protein expression levels of P-c-Raf(Ser259) (0.81 ± 0.14 vs 2.43 ± 0.62), P-GSK-3β(Ser9) (0.49 ± 0.29 vs 1.65 ± 0.56), P-PTEN(Ser380) (0.45 ± 0.14 vs 1.36 ± 0.42), P-PDK1(Ser241) (0.87 ± 0.23 vs 1.78 ± 0.34) and P-AKT(Thr308) (0.93 ± 0.19 vs 2.15 ± 0.62) were significantly lower in LY294002-treated KLF8-overexpressing HCC cells than in DMSO-treated KLF8-overexpressing HCC cells (P < 0.05, n = 3), and the protein expression levels of P-AKT(Ser473) (1.34 ± 0.33 vs 1.62 ± 0.54) and AKT(pan) (1.29 ± 0.47 vs 1.54 ± 0.35) were not significantly different (p > 0.05, n = 3) (Fig. 6a). This evidence concerns the gene GSK3B and hepatocellular carcinoma.