In pcDNA3.1-transfected SMMC7721 cells treated with LY294002 or DMSO, the protein levels of P-AKT (Thr308) were not different, and KLF8-overexpressing HCC cells had higher levels of P-PDK1(Ser241), P-AKT(Thr308) and P-AKT(Ser473). The gene discussed is KLF8; the disease is hepatocellular carcinoma.