To determine the potential of KLF8-overexpressing HCC cells for inducing angiogenesis, SMMC7721 cells transfected with pcDNA3.1 or pcDNA3.1-KLF8 were implanted in chicken embryos, and the angiogenesis induced was then detected in this chicken chorioallantoic membrane (CAM) model. This evidence concerns the gene KLF8 and hepatocellular carcinoma.