To verify our hypothesis that the anticancer mechanism of ACF2 in colorectal cancer is due to CHOP/TRB3/Akt/mTOR-mediated autophagy, CHOP and TRB3 small interference RNA (siRNA) were employed to suppress CHOP and TRB3 expression in HCT116 cells. This evidence concerns the gene AKT1 and colorectal cancer.