To quantitatively evaluate immune cell recruitment and activation in virally treated animals, we evaluated infiltration of macrophages, NK cells, dendritic cells, and CD4 and CD8 positive T-cells within the tumor-bearing brain hemisphere 7 days post HSVQ or HSV-P10 treatment via flow cytometry (gating strategies depicted in Supplementary Figure 6Figure). The gene discussed is CD4; the disease is neoplasm.