Increased FOXO1 and decreased pSerine256-FOXO1 expression were significantly associated with advanced tumor stage and high UICC stage in both EACs (tumor stage: p = 0.0209 and p < 0.0001; UICC stage: p = 0.0201 and p < 0.0001) and ESCCs (tumor stage: p = 0.0003 and p = 0.0016; UICC stage: p = 0.0026 and p = 0.0326). This evidence concerns the gene FOXO1 and neoplasm.