Interference against GPER expression alone (vector shGPER + DMSO) or knockdown of its expression in ZNF32 over-expressing cells (ZNF32 shGPER + DMSO) delayed tumour formation (compared to vector shGPER-NC + DMSO or ZNF32 shGPER-NC + DMSO groups) (Fig. 7b). This evidence concerns the gene GPER1 and neoplasm.