FUT4 and cancer: In addition, we further confirmed the functionality of the designed gRNA sequences by transiently transfecting two different murine cancer cell lines negative for Fut4 and Fut9 gene expression, Panc02 (pancreatic cancer) and LL/2 (Lewis Lung cancer) cells (Supplementary Figure 1C, D, F, G), following exactly the same procedure as mentioned above and again obtained de novo expression of Lewisx.