In addition, we further confirmed the functionality of the designed gRNA sequences by transiently transfecting two different murine cancer cell lines negative for Fut4 and Fut9 gene expression, Panc02 (pancreatic cancer) and LL/2 (Lewis Lung cancer) cells (Supplementary Figure 1C, D, F, G), following exactly the same procedure as mentioned above and again obtained de novo expression of Lewisx. This evidence concerns the gene FUT4 and lung carcinoma.