To further evaluate the effect of ISG15 overexpression on ovarian cancer growth in an immune-competent microenvironment, two mouse ovarian cancer cell lines (ID8 and IG10), which expressed low levels of endogenous ISG15, were transduced with the full-length mouse ISG15 expression construct followed by validation by qPCR (Supplementary Figure S3). Here, ISG15 is linked to ovarian carcinoma.