To evaluate the effect of endogenous ISG15 on ovarian cancer growth in vitro, HGSOC cells OVCA432, which expressed high levels of endogenous ISG15 as confirmed by Western blot analysis (Figure 3A), were transduced with ISG15 specific shRNAs to evaluate the effect of ISG15 silencing on ovarian cancer growth. The gene discussed is ISG15; the disease is ovarian cancer.