Multi-ROC curve analysis was constructed to assess the diagnostic performance of a combination of both AFP (at a cut-off value of 3.5 ng/dl) and lamin B1 (at a 2–ΔΔCq cut-off value of 1.4) to discriminate between patients with HCC and those with CLD. Here, AFP is linked to congenital secretory chloride diarrhea 1.