PROM1 and neoplasm: To determine whether upregulated CD24, EpCAM, Tmprss11e, Fermt1, CDH1 (E-cadherin), and CD133 genes sustain PCA cell proliferation, tumor sphere formation, migration, or invasion, we used lentiviral vector-mediated shRNAs to silence CD24, EpCAM, Tmprss11e, Fermt1, E-cadherin, and CD133 mRNA in PKP PCA cells, selecting stable knockdown clones after two weeks’ puromycin (puro) incubation.