The mean area percentage of collagen fiber deposition and TGF-β immunoexpression for all groups is presented in Figure 6G and Figure 4G. The diabetic nephropathy groups (Group IIa and IIb) and Group III showed a significant increase in the mean area percentage of collagen fiber deposition and TGF-β immunoexpression compared to the control group. The gene discussed is TGFB1; the disease is diabetic kidney disease.