Moreover, we also verified that SOCS3 (suppressor of cytokine signaling 3), one of SOCS3 proteins that were considered to be the main negative regulators of the JAK/STAT signal pathway, was sharply suppressed in PCOS rats, but partially reversed under the treatment of total flavonoids and further demonstrated that total flavonoids played its therapeutic efficiency via regulating the JAK2/STAT3 pathway. This evidence concerns the gene JAK2 and polycystic ovary syndrome.