Here, our data provided that total flavonoids extracted from Nervilia Fordii could recover the estrous cycle, decrease the serum levels of FSH, increase the serum levels of LH, T and INS through partly inhibiting the activation of JAK2/STAT3 pathways and partially regulating the IL-6 and SOCS3 expression in ovaries of PCOS rats. Here, PLOD1 is linked to polycystic ovary syndrome.