In our study, we found that total flavonoids extracted from Nervilia Fordii significantly increased the serum levels of FSH and sharply decreased the serum levels of LH, T and INS levels in PCOS-IR rats, meanwhile high dose total flavonoids could recover estrous cycle in PCOS-IR rats with insulin resistance, indicating the total flavonoids could restore the ovulation function. Here, PLOD1 is linked to Insulin resistance.