In agreement, an independent study used conditional Snail1 and Twist1 knock-out in the pancreatic epithelium of mice, which could spontaneously develop primary pancreatic tumors and metastases to the lung, liver, and spleen due to their Pdx1-Cre-mediated activation of K-Ras and mutant p53 (KPC model) [133]. The gene discussed is TWIST1; the disease is pancreatic neoplasm.