This same study showed that a DENV NS3 peptide, spanning residues 566–585 (corresponding to residues 565–584 in ZIKV NS3, Figure 2A) competitively disrupts the NS3–NS5 interaction, and that a single Asn570Ala substitution abolishes the NS3–NS5 interaction, terminating viral RNA synthesis and infection [31]. This evidence concerns the gene RAF1 and infection.