GDNF expression was strongest in the small intestine of the normal group (39.73 ± 3.87 times that in control mice); after the induction of constipation, GDNF expression decreased in the small intestine of the control group; LB, LF-CQPC03-L, and LF-CQPC03-L all up-regulated GDNF expression in the small intestine of mice with constipation, and the effect of LF-CQPC03-H (27.69 ± 2.61 times that in control mice) on GDNF up-regulation was stronger than that of LB (22.43 ± 2.66 times that in control mice) and LF-CQPC03-L (17.61 ± 2.52 times that in control mice). The gene discussed is GDNF; the disease is constipation disorder.