To investigate the role of spinal inhibitory G-proteins in β-adrenergic receptor agonists-induced hyperglycemia, the mice were pretreated i.t. with PTX (0.1 μg/5 μl) for 6 days, dobutamine (5 μg/5 μl) or terbutaline (10 μg/5 μl) was administered i.t. As shown in Figure 3(A), the plasma insulin level was significantly increased by dobutamine. This evidence concerns the gene INS and Hyperglycemia.