Recently, we have explored an intracoronary TIMP3 delivery route via intracoronary catheter in a pig balloon occlusion model, and demonstrated that the intracoronary delivered TIMP3 can prevent adverse myocardial remodeling in ischemia‐reperfusion injuries.32 To identify a suitable TIMP3 form to improve biological property and function, we have generated multiple glycosylated forms of TIMP3 and fusion constructs. Here, TIMP3 is linked to ischemia reperfusion injury.