ITGAM and neoplasm: Flow cytometry analysis (gating strategy in Figure 2A) showed that the percentage of CD11b+F4/80+Ly6G-Siglec-F- macrophages was significantly increased in tumors of elderly compared with young mice (Figure 2B) and was associated with increasing tumor weight (Figure 2C), i.e., larger tumors have a greater percentage of macrophages.