TNF and hepatocellular carcinoma: Furthermore, the findings showed that HepG2 and HCCLM3 cells treated with combination therapy, APG-1387 with TNF-α or TRAIL, showed increased inhibition of colony formation at 2 weeks when compared with monotherapy, which indicated that the combination of APG-1387 with TNF-α or TRAIL had long-term effects on the inhibition of HCC cell survival and proliferation (Figures 3E,F and Supplementary Figures 6a,b).