To observe the potential impacts of icariin on insulin resistance, C2C12 myotubes were starved serum for 4 h and then treated with or without 0.5 mmol/L of PA in the presence or absence of 25, 50, or 100 μmol/L icariin for 18 h, followed by stimulation with or without 100 nmol/L insulin for 10 min. This evidence concerns the gene INS and Insulin resistance.