These structures were reminiscent of phagocytic pouches previously shown to contain lysosomes (Sierra et al., 2010; Liaury et al., 2012), and with our observations of Ctsz+CD68+ lysosomal and Iba1+ vacuole-like structures in AD brains, and in line with our former flow cytometry demonstration of CD11b+ (CD45+) microglia being loaded with endogenously produced Aβ in Tg but not Wt mice (Babcock et al., 2015). This evidence concerns the gene CTSZ and Alzheimer disease.