It can detect quadriceps sparing myopathy, selective affection of one head of gastrocnemius as of medial head of gastrocnemius and soleus more than lateral head in FKRP (LGMD2I) and calpain-3 deficiency (LGMD2A), and selective affection of lateral head of gastrocnemius as in LGMD2B (dysferlin) [15]. Here, DYSF is linked to autosomal recessive limb-girdle muscular dystrophy type 2I.