To further determine whether ANXA1 regulates autophagy, we established 5–8F NPC cell lines with stable ANXA1 knockdown (5–8F ANXA1 KD), 6–10B NPC cell lines with stable ANXA1 overexpression (6–10B ANXA1 OE) and their respective control cell lines, and then examined the protein levels of BECN1(a fundamental gene for autophagy induction), SQSTM1 and LC3-II, an LC3-phosphatidylethanolamine conjugate and a promising autophagosomal marker (a protein necessary for autophagosome formation)24. Here, MAP1LC3A is linked to nasopharyngeal carcinoma.