In this study, we observed that ANXA1 expression was significantly higher in the NPCs with metastasis than that in the NPCs without metastasis; ANXA1 expression was positively correlated with lymphonode and distant metastasis; ANXA1 OE enhanced while ANXA1 KD reduced NPC cell in vitro migration and invasion and in vivo metastasis. This evidence concerns the gene ANXA1 and nasopharyngeal carcinoma.