To determine whether regulation of autophagy by ANXA1 is involved in such a pathway, we used siRNA to suppress either BECN1 or ATG5 in the ANXA1 KD NPC cells and scramble shRNA control cells, and then examined the levels of LC3-II and EGFP-LC3 puncta. This evidence concerns the gene ANXA1 and nasopharyngeal carcinoma.