To further confirm these increases in the β-catenin, pan-RAS, and EGFR levels by pathologically meaningful APC loss in CRC, we generated RKO cells with the knockout (KO) of APC. The levels of β-catenin, pan-RAS, and EGFR were simultaneously increased (Fig. 1d), and the mRNA levels of the EGFR and CCND1 (cyclin D1) genes, both well-known Wnt/β-catenin signaling response genes, were increased in the APC-KO RKO cells (Fig. 1e). This evidence concerns the gene APC and colorectal carcinoma.